Informace o publikaci

Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions

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ŠEDO Ondrej RADOLFOVÁ-KŘÍŽOVÁ Lenka NEMEC Alexandr ZDRÁHAL Zbyněk

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Microbiological Methods
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Doi http://dx.doi.org/10.1016/j.mimet.2018.10.009
Klíčová slova Acinetobacter spp.; Bacteria profiling; BioTyper; MALDI-TOF MS
Popis A set of 204 taxonomically well-defined strains belonging to 17 Acinetobacter spp., including 11 recently described species (A. albensis, A. bohemicus, A. colistiniresistens, A. courvalinii, A. dispersus, A. gandensis, A. modestus, A. proteolyticus, A. seifertii, A. variabilis, and A. vivianii) and six species of the so-called haemolytic Glade (A. beijerinckii, A. gyllenbergii, A. haemolyticus, A. junii, A. parvus, and A. venetianus), were subjected to MALDI-TOF mass spectrometric profiling. The identification outputs were evaluated using the current version (8.0.0.0) of the commercially available Bruker Daltonics, Biotyper database, which does not contain reference entries for six of the species tested. Up to 29% of the strains were falsely identified as different Acinetobacter spp. present in the Biotyper database, resulting mostly from the close phylogenetic relationship of species of the haemolytic Glade. To obtain more reliable identification, extending the commercial database showed only partial improvement, while the use of an alternative MALDI matrix solution (strongly acidified ferulic acid) allowed correct identification of nearly all problematic strains.
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