Informace o publikaci

Advances in the cellular structural biology of nucleic acids

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GIASSA Ilektra-Chara RYNEŠ Jan FESSL T. TRANTÍRKOVÁ Silvie TRANTÍREK Lukáš

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj FEBS LETTERS
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.13054
Doi http://dx.doi.org/10.1002/1873-3468.13054
Klíčová slova DNA; in-cell chemical probing; in-cell EPR; in-cell NMR; in-cell single-molecule FRET; RNA
Popis Conventional biophysical and chemical biology approaches for delineating relationships between the structure and biological function of nucleic acids (NAs) abstract NAs from their native biological context. However, cumulative experimental observations have revealed that the structure, dynamics and interactions of NAs might be strongly influenced by a broad spectrum of specific and nonspecific physical-chemical environmental factors. This consideration has recently sparked interest in the development of novel tools for structural characterization of NAs in the native cellular context. Here, we review the individual methods currently being employed for structural characterization of NA structure in a native cellular environment with a focus on recent advances and developments in the emerging fields of in-cell NMR and electron paramagnetic resonance spectroscopy and in-cell single-molecule FRET of NAs.
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