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The plant Pontin and Reptin homologues, RuvBL1 and RuvBL2a, colocalize with TERT and TRB proteins in vivo, and participate in telomerase biogenesis

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SCHOŘOVÁ Šárka FAJKUS Jiří ZÁVESKÁ DRÁBKOVÁ Lenka HONYS David PROCHÁZKOVÁ SCHRUMPFOVÁ Petra

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj The Plant Journal
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www Webová stránka publikace v časopise
Doi http://dx.doi.org/10.1111/tpj.14306
Klíčová slova Telomerase assembly; Pontin; Reptin; AtTERT; AtTRB; AtRuvBL; AtPOT1a; nucleolus; Arabidopsis.
Popis Telomerase maturation and recruitment to telomeres is regulated by several telomerase- and telomereassociated proteins. Among a number of proteins, human Pontin and Reptin play critical roles in telomerase biogenesis. Here we characterized plant orthologues of Pontin and Reptin, RuvBL1 and RuvBL2a, respectively, and show association of Arabidopsis thaliana RuvBL1 (AtRuvBL1) with the catalytic subunit of telomerase (AtTERT) in the nucleolus in vivo. In contrast to mammals, interactions between AtTERT and AtRuvBL proteins in A. thaliana are not direct and they are rather mediated by one of the Arabidopsis thaliana Telomere Repeat Binding (AtTRB) proteins. We further show that plant orthologue of dyskerin, named AtCBF5, is indirectly associated with AtTRB proteins but not with the AtRuvBL proteins in the plant nucleus/nucleolus, and interacts with the Protection of telomere 1 (AtPOT1a) in the nucleolus or cytoplasmic foci. Our genomewide phylogenetic analyses identify orthologues in RuvBL protein family within the plant kingdom. Dysfunction of AtRuvBL genes in heterozygous T-DNA insertion A. thaliana mutants results in reduced telomerase activity and indicate the involvement of AtRuvBL in plant telomerase biogenesis.
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