Informace o publikaci

Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells

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SKUBALOVA Zuzana MICHALKOVA Hana MICHALEK Petr STRMISKA Vladislav GURAN Roman RODRIGO Miguel Angel Merlos CASTKOVA Klara HYNEK David PEKAŘÍK Vladimír ZITKA Ondrej ADAM Vojtech HEGER Zbynek

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj Colloids and Surfaces B: Biointerfaces
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www http://dx.doi.org/10.1016/j.colsurfb.2019.110391
Doi http://dx.doi.org/10.1016/j.colsurfb.2019.110391
Klíčová slova Cytotoxicity; Nanotoxicology; Oxidative stress; Proteomics; Titanium
Popis Nanoparticular form of titanium dioxide (TiO2 NPs) belongs to important industrial material. Despite being widely used, serious contradictions regarding biosafety of TiO2 NPs remain. We anticipate that such discrepancies could be due to a lack of understanding of a linkage between TiO2 NPs phase composition and cytotoxicity. Therefore, we synthesized two types of biphasic TiO2 NPs differing in an anatase-brookite phase composition. The study presents an array of in vitro data suggesting that TiO2 NPs with a prevailing anatase phase composition possess higher cytotoxicity compared to TiO2 NPs with an equal anatase-brookite crystallinity. This phenomenon was evidenced by significantly higher inhibition of metabolic activity and growth of epithelial and neuroblast-like cells. Moreover, anatase-prevailing TiO2 NPs tend to produce higher amount of reactive oxygen species resulting in DNA fragmentation. Further insights into the molecular aspects of cytotoxicity of anatase-prevailing TiO2 NPs were obtained by comparative proteomics delineating that TiO2 NPs deregulate expression of a variety of proteins and associated pathways. This inevitably results in a decreased cellular ability to detoxify reactive oxygen species and respond to various stress conditions. The study provides novel data that add another piece to the jigsaw of the relation between structural features of NPs and biosafety.
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