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In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes

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KRAFČÍKOVÁ Michaela HANSEL-HERTSCH R. TRANTÍREK Lukáš TRANTÍRKOVÁ Silvie

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25
Doi http://dx.doi.org/10.1007/978-1-4939-9666-7_25
Klíčová slova In-cell NMR; Xenopus laevis oocyte; DNA; RNA; G-quadruplex
Popis G-quadruplexes are inherently polymorphic nucleic acid structures. Their folding topology depends on the nucleic acid primary sequence and on physical-chemical environmental factors. Hence, it remains unclear if a G-quadruplex topology determined in the test tube (in vitro) will also form in vivo. Characterization of G-quadruplexes in their native environment has been proposed as an efficient strategy to tackle this issue. So far, characterization of G-quadruplex structures in living cells has relied exclusively on the use of Xenopus laevis oocytes as a eukaryotic cell model system. Here, we describe the protocol for the preparation of X. laevis oocytes for studies of G-quadruplexes as well as other nucleic acids motifs under native conditions using in-cell NMR spectroscopy.
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