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Multichannel separation device with parallel electrochemical detection

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KOMENDOVÁ Martina NAWADA Suhas METELKA Radovan SCHOENMAKERS Peter J. URBAN Jiří

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Chromatography A
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://doi.org/10.1016/j.chroma.2019.460537
Doi http://dx.doi.org/10.1016/j.chroma.2019.460537
Klíčová slova 3D printing; Multi-channel device; Dopamine metabolism; Electrochemical detection; Polymer monoliths; Titanium
Popis A device with four parallel channels was designed and manufactured by 3D printing in titanium. A simple experimental setup allowed splitting of the mobile phase in four parallel streams, such that a single sample could be analysed four times simultaneously. The four capillary channels were filled with a monolithic stationary phase, prepared using a zwitterionic functional monomer in combination with various dimethacrylate cross-linkers. The resulting stationary phases were applicable in both reversed-phase and hydrophilic-interaction retention mechanisms. The mobile-phase composition was optimized by means of a window diagram so as to obtain the highest possible resolution of dopamine precursors and metabolites on all columns. Miniaturized electrochemical detectors with carbon fibres as working electrodes and silver micro-wires as reference electrodes were integrated in the device at the end of each column. Experimental separations were successfully compared with those predicted by a three-parameter retention model. Finally, dopamine was determined in human urine to further confirm applicability of the developed device.
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