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First records raise questions: DNA barcoding of Odonata in the middle of the Mediterranean

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REWICZ Tomasz MÓRA Arnold TOŃCZYK Grzegorz SZYMCZAK Ada GRABOWSKI Michal CALLEJA Eman J. PERNECKER Balint CSABAI Zoltán Szabolcs

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Genome
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://doi.org/10.1139/gen-2019-0226
Doi http://dx.doi.org/10.1139/gen-2019-0226
Klíčová slova Malta; islands; COI; BIN; species inventory; DNA barcode reference library
Popis We present the results of the first-ever DNA barcoding study of odonates from the Maltese Islands. In total, 10 morphologically identified species were collected during a two-week long expedition in 2018. Eighty cytochrome c oxidase subunit I (COI) barcodes were obtained from the collected specimens. Intra- and interspecific distances ranged from 0.00% to 2.24% and 0.48% to 17.62%, respectively. Successful species identification based on ascribing a single morphological species to a single Barcode Index Number (BIN) was achieved for eight species (80%). In the case of two species, Ischnura genei and Anax parthenope, BINs were shared with other closely related species. The taxonomic status of I. genei is questionable and the phylogenetic relationship between A. imperator/parthenope is not clear. Further studies involving a series of adult specimens collected in a wide spatial range and nuclear markers are necessary to resolve these cases. Therefore, this dataset serves as an initial DNA barcode reference library for Maltese odonates, within a larger project: Aquatic Macroinvertebrates DNA Barcode Library of Malta.

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