Informace o publikaci
The effect of beta-cyclocitral treatment on the carotenoid content of transgenic Marsh grapefruit (Citrus paradisi Macf.) suspension-cultured cells
| Autoři | |
|---|---|
| Rok publikování | 2020 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Phytochemistry |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://www.sciencedirect.com/science/article/pii/S0031942220308281?via%3Dihub |
| Doi | http://dx.doi.org/10.1016/j.phytochem.2020.112509 |
| Klíčová slova | Citrus paradis; Rutaceae; Citrus; Suspension-cultured cells; Chemical elicitor; beta-yclocitral; Carotenoid; Provitamin A |
| Popis | This work reports the development of suspension culture system of transgenic Marsh grapefruit (Citrus paradisi Macf., Rutaceae) callus overexpressing bacterial phytoene synthase; and the use of this suspension culture to investigate the effects of beta-cyclocitral on carotenoid content and composition. At a beta-cyclocitral concentration of 0.5 mM and after ten days cultivation, analysis of the camtenoids showed a significant increase in the content of beta-, alpha-carotene, and phytoene predominantly. The maximal increase in total provitamin A camtenoids content following beta-cyclocitral application was similar to 2-fold higher than the control, reaching 245.8 mu g/g DW. The trend for increased transcript levels of biosynthetic genes PSY and ZDS correlated with the enhancement of the content of these carotenes following beta-cyclocitral treatment and GC-MS based metabolite profiling showed significant changes of metabolite levels across intermediary metabolism. These findings suggest that beta-cyclocitral can act as a chemical elicitor, to enhance the formation of carotenes in citrus suspension-cultured cells (SCC), which could be utilized in studying the regulation of carotenoid biosynthesis and biotechnological application to the renewable production of nutritional carotenoids. |