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Digital PCR can provide improved BCR-ABL1 detection in chronic myeloid leukemia patients in deep molecular response and sensitivity of standard quantitative methods using EAC assays

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SMITALOVÁ Dagmar DVOŘÁKOVÁ Dana RÁČIL Zdeněk ROMŽOVÁ Marianna

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Practical Laboratory Medicine
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Klíčová slova Chronic myeloid leukemia; BCR-ABL1 monitoring; GeneXpert BCR-ABL Monitor assay; RT-qPCR; Digital PCR
Popis BCR-ABL1 molecular detection using quantitative PCR (qPCR) methods is the golden standard of chronic myeloid leukemia (CML) monitoring. However, due to variable sensitivity of qPCR assays across laboratories, alternative methods are tested. Digital PCR (dPCR) has been suggested as a robust and reproducible option. Here we present a comparison of droplet dPCR with routinely used reverse-transcription qPCR (RT-qPCR) and automated GeneXpert systems. Detection limit of dPCR was above 3 BCR-ABL1 copies, although due to background amplification the resulting sensitivity was 0.01% BCR-ABL1 (MR4.0). Nevertheless, in comparison with GeneXpert, dPCR categorized more than 50% of the patients into different MR groups, showing a potential for improved BCR-ABL1 detection.
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