Informace o publikaci

Lyn Phosphorylates and Controls ROR1 Surface Dynamics During Chemotaxis of CLL Cells

Logo poskytovatele
Logo poskytovatele
Logo poskytovatele
Logo poskytovatele
Logo poskytovatele
Logo poskytovatele
Autoři

DAVE Zankruti VONDÁLOVÁ BLANÁŘOVÁ Olga ČADA Štěpán JANOVSKÁ Pavlína ZEZULA Nikodém BĚHAL Martin HANÁKOVÁ Kateřina GANJI Sri Ranjani KREJČÍ Pavel GÖMÖRYOVÁ Kristína PESCHELOVÁ Helena ŠMÍDA Michal ZDRÁHAL Zbyněk PAVLOVÁ Šárka KOTAŠKOVÁ Jana POSPÍŠILOVÁ Šárka BRYJA Vítězslav

Rok publikování 2022
Druh Článek v odborném periodiku
Časopis / Zdroj Frontiers in Cell and Developmental Biology
Citace
www https://www.frontiersin.org/articles/10.3389/fcell.2022.838871/full
Doi http://dx.doi.org/10.3389/fcell.2022.838871
Klíčová slova Ror1; Lyn; crosstalk; phosphorylation; CLL; BCR; signaling pathway
Popis Chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL) are malignancies characterized by the dependence on B-cell receptor (BCR) signaling and by the high expression of ROR1, the cell surface receptor for Wnt-5a. Both, BCR and ROR1 are therapeutic targets in these diseases and the understanding of their mutual cross talk is thus of direct therapeutic relevance. In this study we analyzed the role of Lyn, a kinase from the Src family participating in BCR signaling, as a mediator of the BCR-ROR1 crosstalk. We confirm the functional interaction between Lyn and ROR1 and demonstrate that Lyn kinase efficiently phosphorylates ROR1 in its kinase domain and aids the recruitment of the E3 ligase c-CBL. We show that ROR1 surface dynamics in migrating primary CLL cells as well as chemotactic properties of CLL cells were inhibited by Lyn inhibitor dasatinib. Our data establish Lyn-mediated phosphorylation of ROR1 as a point of crosstalk between BCR and ROR1 signaling pathways.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info