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Increased plasma cortisol level as acute response to glochidia parasitism

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REICHARD Martin DOUDA Karel BLAŽEK Radim JANOVSKÁ Anna

Rok publikování 2023
Druh Článek v odborném periodiku
Časopis / Zdroj Environmental Biology of Fishes
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://doi.org/10.1007/s10641-022-01379-6
Doi http://dx.doi.org/10.1007/s10641-022-01379-6
Klíčová slova Cost of parasitism; Freshwater bivalve; Glochidiosis; Hydrocortisone; Plasma cortisol; Unionida
Popis Acute stress response in the European bitterling, Rhodeus amarus, to glochidia parasitism by the invasive unionid mussel, Sinanodonta woodiana, was quantified by analysing cortisol plasma levels using liquid chromatography coupled to mass spectrometer. We tested a novel method which required as little as 4 mu L of plasma by increasing the volume using charcoal-treated plasma. Bitterling were observed to increase cortisol levels significantly in response to glochidia infestation. Overall, this technique allows the precise measurement of steroid hormone plasma concentrations in small fish.

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