Informace o publikaci
MODIFICATION OF CHEMILUMINESCENCE SYSTEM FOR BLOOD SPOT DETECTION
| Autoři | |
|---|---|
| Rok publikování | 2024 |
| Druh | Konferenční abstrakty |
| Citace | |
| Popis | The work focused on improving the luminol chemiluminescence system, often used in forensic science to detect blood spots. In the experimental part, the emission parameters of various modified luminol systems were investigated. These results were used to optimize the chemiluminescence conditions of the luminol mixture. Identical measurements were also performed on Bluestar tablets, which are now the established standard for blood spot detection. The results obtained led to the design of an optimal system for chemiluminescence detection of blood spots. As part of the effort to improve the system, the CRET (Chemiluminescent Resonance Energy Transfer) method was investigated on fluorescent substances (fluorescein, eosin, rhodamine 6G, rhodamine B and rhodamine 110) as a potential means to increase sensitivity and detection efficiency. In addition, efforts were made to prevent luminescence quenching due to the substitution of solvent (H2O) for deuterated water (D2O). The proposed improvements were subsequently tested and compared on synthetic blood spots. |