Informace o publikaci
Screening of Glycyrrhiza species for anti-inflammatory phenols
| Autoři | |
|---|---|
| Rok publikování | 2024 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Roots and rhizomes of Glycyrrhiza species (Fabaceae) have long been used for their antiinflammatory, expectorant, and antimicrobial effects. Our research is dedicated to screening and selection of different Glycyrrhiza taxa for further isolation of bioactive compounds with with an emphasis on prenylated phenols. Plant material was obtained by cooperation with Mendel University, Faculty of Horticulture. Raw material from total of thirteen Glycyrrhiza varieties including six species (G. glabra, G. uralensis, G. pallidiflora, G. foetida, G. yunnanensis, and G. echinata) was extracted with ethanol. Extracts were fractionated by liquidliquid extraction (n-hexane, chloroform, ethyl acetate and water). All fractions were then analysed by HPLC-DAD and ELSD. Chloroform and ethyl acetate fractions were tested for cytotoxicity and inhibition of NF?B on THP-1 cells. The major compounds from G. uralensis were isolated by flash chromatography and purified by semipreparative HPLC. HPLC-DAD analysis has not revealed any apparent intraspecific difference in content of phenolic compounds. Structures of isolated compounds will be elucidated by various spectral methods. Identified compounds will undergo further bioassays for anti-inflammatory effects. |
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