Informace o publikaci
Development of in vitro cultures from Astragalus thracicus Griseb and Astragalus aitosensis (Ivanisch.) and evaluation of their bioproduction potential
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | With more than 2200 taxonomically classified species, genus Astragalus (Fabaceae) is one of the largest genera of dicotyledonous plants. Astragalus species are widespread mainly in the temperate regions of the northern hemisphere, growing in arid and semiarid regions up to 1700 meters above sea level. Nearly 133 species grow in Europe, of which 29 in Bulgaria. Several of them are endemic and thus included in the Red List of Bulgaria under vulnerable, endangered or critically endangered status. In this research we investigated the peculiarities in establishment and development of in vitro cultures of two endangered Astragalus species - A. thracicus and A. aitosensis. Astragalus thracicus (Griseb) is a tertiary relict and Balkan endemic species. Nowadays it is found only in three habitats in Bulgaria, along it could be found in some limited areas of Greek and Turkish regions of Thrace. Astragalus aitosensis (Ivan.) is an endemic relic plant in Bulgaria. Nowadays the plant is found only in the suburbs of Aytos, a small Bulgarian town. In a series of in vitro experiments, we investigated and optimized the basic growth conditions, suitable for growing and maintaining of stable and long-lasting callus cultures. The parameters, which we investigated were: effect of scarification over the seed germination rate, type and concentration of gelling agent, hormone regiments included, type and concentration of carbon sources. Additionally, we measured behaviour and flavonoid biosynthetic potential of callus cultures after stress induction with elicitors like methyl jasmonate (MeJ), salicylic acid (SA) and yeast extract (YE). In case of biotic stress, we concluded that growth index of callus cultures from A. thracicus decreased inversely proportional to the concentration of elicitors applied, as SA showed the strongest growth-inhibiting effect. Synthetic potential of the same callus cultures was evaluated according HPLC/MS measure of three flavonol aglycons – kaempferol, quercetin and methylquercetin. The highest amount of kaempferol (9.12 µg/g dry weight) and quercetin (5.72 µg/g dry weight) was detected when callus cultures were exposed for 72 hours in medium supplemented with 50 mg/l YE. For the synthesis of the third aglycon, methylquercetin, the best inductive properties (equivalent to 4.69 µg/g dry weight) were measured in media supplemented with 200 µmol/l MeJ for 72 hours. The fastest kaempferol synthesis was found at 24 hours in the medium supplemented with 1000 µmol/l SA, quantified as 7.61 µg/g dry weight. Based on all these experiments, the study resulted with developing of a protocol for micropropagation. Acnowledgements: Czech Science Foundation Project no. GA23-04655S Role of prenylation and glycosylation patterns in anti-inflammatory activity and metabolism of natural phenolic compounds |