Informace o publikaci

Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and Pemetrexed

Autoři	OZ Umut Can KUCUKTURKMEN Berrin GOMEZ I Jennifer ELSHERBENY Amr TEKNECI Seda Ipek ESIM Ozgur GOKSEVER Selin OZKOSE Umut Ugur GULYUZ Sevgi BAZAN-COBELO Claudia YILMAZ Ozgur USTUNDAG Aylin MEDALOVÁ Jiřina BOZKIR Asuman ZAJÍČKOVÁ Lenka ER Engin
Rok publikování	2025
Druh	Recenzovaný odborný článek
Časopis / Zdroj	ACS Omega
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	https://pubs.acs.org/doi/10.1021/acsomega.5c07253?ref=PDF
Doi	https://doi.org/10.1021/acsomega.5c07253
Klíčová slova	Cancer; Drug delivery; Hydrodynamics; Nanoparticles; Quantum dots
Popis	The simultaneous delivery of therapeutic agents and imaging probes using polymeric nanoparticles (NPs) has gained significant attention for cancer treatment. In this work, we developed a multifunctional nanocarrier system composed of an amphiphilic block copolymer, poly(2-ethyl-2-oxazoline)-b-poly(?-caprolactone) (PEtOx-b-PCL), and dimethyldidodecylammonium bromide (DDAB), for the codelivery of the chemotherapeutic drug pemetrexed (PMT) and nitrogen- or sulfur-doped graphene quantum dots (N-GQDs or S-GQDs) as fluorescent probes. Critical formulation parameters were optimized using a central composite design (CCD). The optimized NPs exhibited favorable physicochemical properties, including positive surface charge (6–8 mV), hydrodynamic diameters of ~140 nm, and high encapsulation efficiency for both PMT (46–56%) and GQDs (>98%). In vitro assays revealed that PMT-loaded nanoparticles (NPs) significantly enhanced cytotoxicity against MCF-7 cells. At a concentration of 2 ppm after 72 h, N-PMT NPs and S-PMT NPs inhibited cell proliferation by 50.7% and 53.8%, respectively, compared to 37.8% inhibition with free PMT at the same dose. Confocal microscopy confirmed efficient intracellular uptake and strong fluorescence signals, supporting their potential for bioimaging. Collectively, these results demonstrate that this two-in-one nanocarrier system significantly enhances chemotherapeutic efficacy while enabling real-time imaging, establishing a promising platform for drug delivery and noninvasive treatment monitoring in cancer nanomedicine.