Informace o publikaci
Anti-biofilm and anti-quorum sensing activity of galloylquinic acids from Copaifera lucens leaves against clinical isolates of multidrug-resistant Pseudomonas aeruginosa in open wound infection: in vitro and in vivo study
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | This presentation investigates the antibacterial, anti-biofilm, and anti-quorum sensing actions of galloylquinic acid compounds (GQAs) derived from the brazillian Copaifera lucens leaves against clinical isolates of multidrug- resistant (MDR) Pseudomonas aeruginosa. Our results showed significant inhibition zone diameters (25-40 mm) in the antibacterial activity assay, with minimal inhibitory concentrations (MIC) ranging from 1-4 µg/mL and minimal bactericidal concentrations (MBC) between 2-16 µg/mL. GQAs effectively interfered with planktonic P. aeruginosa isolates and inhibited their growth within pre-formed biofilms, with MBIC80 and MBEC80 values of 64 µg/mL and 128 µg/mL, respectively. Fluorescence staining and confocal microscopy revealed a substantial reduction in cell viability and biofilm thickness (62.5%) following exposure to 128 µg/mL of GQAs. Scanning electron micrographs further confirmed GQAs ability to disrupt biofilm and bacterial structures by interfering with biomass and exopolysaccharides (Fig. 1). Moreover, GQAs significantly (p<0.05) reduced the production of virulence factors and bacterial motility, including rhamnolipid, pyocyanin, as well as swarming and swimming motility. This reduction is attributed to GQAs downregulation (up to 89%) of quorum-sensing genes (lasI, lasR, pqsA, and pqsR) involved in biofilm formation. In conclusion, GQAs derived from C. lucens represent promising antibiofilm agents with the potential to enhance wound healing in biofilm-associated infections caused by MDR P. aeruginosa clinical isolates. |