Informace o publikaci
DEVELOPMENT OF NOVEL FLUOROPHORE DERIVATIVES FOR GLYCAN ANALYSIS
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Protein glycosylation and the impact of glycan structures on their function represent a crucial area of research in disease diagnostics and the analysis of pathogenic mechanisms. However, glycan analysis is challenging due to their structural variability and the limited sensitivity of existing analytical methods. Therefore, a combination of fluorescence detection and mass spectrometry (MS) is often employed [1]. We focused on the development of novel fluorophore derivatives containing sulfonic and cationic groups, enhancing their solubility in aqueous solutions and improving glycan detection sensitivity. These modifications are based on established skeletal structures such as BODIPY. Additionally, a hydrazine moiety is incorporated to facilitate stable binding with oligosaccharides through hydrazone chemistry. This approach offers higher reactivity with carbonyl-containing carbohydrate compounds, ensuring the formation of stable complexes suitable for fluorescence detection and MS analysis. The hydrazinefunctionalized fluorophores are synthesized via "click" chemistry to ensure compatibility and synthetic efficiency. The developed labeling methods provide an innovative approach for more sensitive and accurate glycan analysis, contributing to a better understanding of biological processes and disease diagnostics. |
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