Informace o publikaci
Porovnání metod detekce bakterií Leptospira interrogans s.l. u malých savců
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Další prezentace na konferencích |
| Citace | |
| Popis | Leptospira spp. are pathogenic spirochetes causing leptospirosis, a zoonosis with 20–30 human cases annually in the Czech Republic. Small mammals, mainly rodents, are key reservoirs. This study aimed to detect antibodies against Leptospira serovars in wild small mammals from the Moravian Karst and to assess infection risk for humans. Between 2021–2024, 171 small mammals from eight species were trapped. Organ rinses were tested for antibodies using the microscopic agglutination test (MAT, gold standard) and ELISA. Nine serovars occurring in the Czech Republic (Bratislava, Canicola, Grippotyphosa, Icterohaemorrhagiae, Istrica, Pomona, Sejroe, Sorex Jalna, Tarassovi) served as antigens. Samples were diluted 1:100. MAT detected antibodies in 29.8% (51/171), mainly Grippotyphosa (11.1%), Istrica (10.5%), Icterohaemorrhagiae (8.2%), Bratislava (6.4%), Sejroe (2.3%), and rarely Canicola, Pomona, and Sorex Jalna (0.6% each). ELISA confirmed positivity in 25.7% (44/171). Concordance between methods was low (33.9%, ? = 0.041). Compared to earlier South Moravia results (6.1% positivity, only Pomona and Grippotyphosa), prevalence was higher and serovar diversity broader. Organ rinses appear more sensitive than heart imprints. This study shows a relatively high prevalence of Leptospira antibodies in small mammals of the Moravian Karst, highlighting zoonotic risk. Future work will include PCR to verify pathogen persistence. Acknowledgement: Supported by Specific Research MUNI/A/1258/2022. References: Adler B, de la Pena Moctezuma A. Vet Microbiol. 2010;140:287–296. Haake DA, Levett PN. Curr Top Microbiol Immunol. 2015;387:65–97. Levett PN. Clin Microbiol Rev. 2001;14:296–326. Žákovská A, et al. Vector Borne Zoonotic Dis. 2022;22:123–130. |