Informace o publikaci
Immunofluorescence distribution of atypical chemokine receptor ACKR3/CXCR7 in dorsal root ganglion neurons of a mouse model of neuropathic pain
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| Rok publikování | 2025 |
| Druh | Další prezentace na konferencích |
| Citace | |
| Popis | Under physiological conditions, primary sensory neurons of the dorsal root ganglia (DRG) display a balanced expression of endogenous opioid peptides and receptors, including the delta opioid receptor (DOR). The chemokine receptor CXCR7, also known as the atypical chemokine receptor ACKR3, was recently identified as an atypical opioid receptor. However, its intraneuronal localization and dynamics in DRG neurons remain unexplored in experimental models of neuropathic pain. We used a mouse spared nerve injury model with unilateral tibial nerve sparing (SNIt) to study the intraneuronal immunodetection of the CXCR7 protein in DRG neurons, compared to naive and sham-operated animals. Double immunofluorescence staining for DOR and CXCR7, combined with confocal microscope analysis, was utilized to investigate their potential intraneuronal interactions. Immunofluorescence and confocal microscopy analyses revealed CXCR7 immunofluorescence (IF) predominantly in the neuronal cytoplasm of the DRG in naive, sham- and SNIt-operated mice. On post-operation day (POD) 7 and 21, both sham and SNIt operations induced a bilateral increase in cytoplasmic CXCR7-IF intensity in small- and medium-sized neurons, accompanied by a shift of CXCR7-IF toward the neuronal surface. In contrast, large-sized neurons exhibited only an increase in intraneuronal CXCR7-IF intensity. CXCR7-IF was colocalized with DOR-IF in vesicular structures within the neuronal cytoplasm, as observed by confocal microscopy. These results suggest a mechanism of modulation in endogenous and exogenous potential DOR ligands in DRG neurons in an experimental neuropathic pain model. |
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