Informace o publikaci
Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | ACTA VETERINARIA BRNO |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://actavet.vfu.cz/94/4/0317/ |
| Doi | https://doi.org/10.2754/avb202594040317 |
| Klíčová slova | total visible count; heat treatment; hbl genes; nhe genes; ces gene; Bacillus cytotoxicus |
| Popis | The aim of this study was to monitor the growth of Bacillus cereus in 11 different types of naturally contaminated ready-to-cook plant-based meat analogues (PBMAs). The PBMAs were prepared according to the manufacturer's instructions and subsequently stored at room temperature for up to 24 h. Bacillus spp. represented the second most numerous group of microbiota detected in PBMAs after lactic acid bacteria. Bacillus cereus sensu lato (s.l.) was isolated from all 11 PBMA samples. Following heat treatment and 24-h storage at 22 degrees C, B. cereus s.l. was detected by plate counting in five samples, with one product reaching nearly 4 log CFU/g. The presence of B. cereus sensu stricto (s.s.) was detected in all 11 samples. Bacillus thuringiensis was present in 7 samples, and in 4 PBMA samples the species B. weihenstephanensis, B. mycoides, andB. cytotoxicus were found. Atotal of 95.7% ofB. cereus s.l. strains contained genes for toxin production. The presence of nhe genes was demonstrated in 92.5%, hbl genes in 59.0% of the analysed B. cereus s.l. strains, the cytK-1 gene in 5 strains (B. cytotoxicus). The presence of the ces gene was confirmed in 5% of B. cereus s.l. strains. In total, 47 different combinations of genes for toxin production were detected in B. cereus s.l. strains. The almost 100% presence of virulence genes associated with the possibility ofB. cereus growth during inappropriate storage of PBMAs thus poses ariskforthe development of foodborne diseases. |
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