Informace o publikaci
Identification of Plant Phenolics from Paulownia tomentosa and Morus alba as Novel PPARγ Partial Agonists and Hypoglycemic Agents
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Recenzovaný odborný článek |
| Časopis / Zdroj | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://pubs.acs.org/doi/10.1021/acs.jafc.4c11398?src=getftr&utm_source=clarivate&getft_integrator=clarivate |
| Doi | https://doi.org/10.1021/acs.jafc.4c11398 |
| Klíčová slova | diabetes mellitus; hypoglycemic; natural products; plant phenolics; PPAR gamma |
| Popis | The aim of our study was to determine the PPAR gamma agonism and hypoglycemic activity of natural phenolics isolated from Paulownia tomentosa and Morus alba. We started with a molecular docking preselection, followed by in vitro cell culture assays, such as PPAR gamma luciferase reporter gene assay and PPAR gamma protein expression by Western blot analysis. The ability of the selected compounds to induce GLUT4 translocation in cell culture and lower blood glucose levels in chicken embryos was also determined. Among the thirty-six plant phenolic compounds, moracin M showed the highest hypoglycemic effect in an in ovo experiment (7.33 +/- 2.37%), followed by mulberrofuran Y (3.84 +/- 1.34%) and diplacone (3.69 +/- 1.37%). Neither moracin M nor mulberrofuran Y showed a clear effect on the enhancement of GLUT4 translocation or agonism on PPAR gamma, while diplacone succeeded in both (3.62 +/- 0.16% and 2.4-fold +/- 0.2, respectively). Thus, we believe that the compounds moracin M, mulberrofuran Y, and diplacone are suitable for further experiments to elucidate their mechanisms of action. |
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