Dual-Organoid Biosensor for Monitoring Cardiac Conduction Disturbances In Vitro

• Autoři: MÁČALA Jakub; SVĚRÁK Filip; KABANOV Daniil; VRANA Šimon; BECKEROVÁ Deborah; MÁCHAL Jan; PEŠL Martin; ROTREKL Vladimír; PŘIBYL Jan
• Rok publikování: 2025
• Druh: Konferenční abstrakty
• Fakulta / Pracoviště MU: Středoevropský technologický institut

Přiložené soubory

• sbornik_2025.pdf

• Popis: Atomic Force Microscopy (AFM) is traditionally employed as a high-resolution imaging tool to analyze surface morphology and mechanical properties by scanning a sharp tip mounted on a flexible cantilever. Robust feedback system and micromechanical transducers are responsible for the constant adjustment of tip-sample interaction; however, it can be utilized to monitor the contraction dynamics of cardiomyocytes. Moreover, by distinguishing vertical and lateral contractile movements, AFM enables precise differentiation between focal and conductive arrhythmic contractions. This presentation introduces an AFM-based biosensor using a dual-beating human pluripotent stem cell (hPSC)-derived organoid. The high biosensor sensitivity allows detailed analysis of contractile behavior under pharmacological modulation with cardiomodulating drugs. The dual-organoid system enhances model robustness by minimizing variability inherent 19 in single-cell studies, thereby improving its translational relevance for cardiotoxicity assessment.

Související projekty

• CIISB - Česká infrastruktura pro integrativní strukturní biologii
• Machine Learning in Nanomaterial Biocompatibility Assessment
• Mechanické vlastnosti měkkých materiálů v nanorozměrech