Informace o publikaci
Beyond antagonism: Phage–mupirocin treatment broadens the spectrum of Staphylococcus aureus decolonization
| Autoři | |
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| Rok publikování | 2026 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Objective: The production of Panton-Valentin leukocidin (PVL) by S. aureus strains is associated with chronic or recurrent infections, which can lead to complications such as surgical site infections. Therefore, mupirocin decolonization is often indicated, which can lead to the emergence of resistance. Methods: We investigated the coadministration of Kayvirus and mupirocin to prevent resistance and broaden the antimicrobial action. To obtain PVL strains with varying levels of mupirocin resistance, we used lysogenization and adaptive laboratory evolution. Results: Of the 37 PVL-encoding S. aureus isolates from wound samples, 11% were phage-resistant, but all were susceptible to mupirocin. Strains with low and high levels of mupirocin resistance were prepared and verified. We assessed the growth of strains with different susceptibility profiles and quantified mupirocin-phage interactions, which were mostly inhibitory due to impaired proteosynthesis. Conclusion: In mupirocin-susceptible strains, the phage was inhibited by the mupirocin-host tRNA synthetase interaction, while mupirocin efficacy was unaffected. In mupirocin-resistant phage-susceptible strains, the altered or alternative synthetase allowed protein synthesis in the presence of mupirocin. This enabled effective phage infection, leading to the eradication of high-level-resistant strains and preventing the emergence of low-level mupirocin resistance. Thus, the combination of mupirocin with Kayvirus broadens the spectrum of strains susceptible to treatment. |
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