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Chromatographic purification and separation of plasmid and lambda phage DNAs

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RITTICH Bohuslav ŠPANOVÁ Alena SKALNÍKOVÁ Magdalena

Druh Článek ve sborníku
Konference 22nd International Symposium on the Separation of Proteins, Peptides and Polynucleotides
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Biotechnologie a bionika
Klíčová slova Plasmid and phage DNAs; purification; chromatography
Popis Plasmid pBR322 DNA was purified by gel permeation chromatography. Recovered plasmid DNA was free of RNA and retained its biological activity. The biological activity of purified plasmid DNA was tested by electrotransformation into bacterial cells. DNA fragments of lambda DNA and lambda DNA were separated on column for gel permeation chromatography. Small fragments were eluted in void volume; large fragments and lambda DNA were separated in reversed order than expected according to GPC and according to the slalom chromatography mechanisms.
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