Informace o publikaci

Surface immobilized protein multilayers for cell seeding

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DVOŘÁK Petr BRYNDA E. PACHERNÍK Jiří HOUSKA M. PIENTKA Z.

Rok publikování 2005
Druh Článek v odborném periodiku
Časopis / Zdroj Langmuir
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Obor Genetika a molekulární biologie
Klíčová slova MOUSE EMBRYOS; BIOSENSORS; ALBUMIN; HEPARIN; INVITRO; PLASMA
Popis The adhesion and growth of mouse embryonic stem cells line D3 were tested in polystyrene culture dishes coated with the multilayer assemblies containing various cell-adhesive proteins such as gelatin, collagen IV, and laminin or polycations polylysine and poly(ethyleneimine). The cells were seeded in complete serum-containing media or in serum-free media and in the presence (non-differentiated) and/or absence (differentiated) of leukemia inhibitory factor. The cells grew best on surfaces coated with gelatin and collagen IV assemblies. There were no significant differences in the growth of the non-differentiated and differentiated cells in complete serum-containing media. When seeded in serum-free media, non-differentiated cells grew better than the differentiated ones. The layer-by-layer deposition appears to be a practicable technique by which scaffolds for tissue engineering can be coated with biomolecular assemblies tailored to specific cells and applications.