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Proximal and distal nerve injuries induce changes of endoneurial laminin-1 in the spinal nerve roots - A quantitative immunohistochemical assessment

Název česky Proximální a distální poškození nervu vyvolávají v kořenach spinálního nervu změny endoneuriálního lamininu 1 - Kvantitativní imunohistochemické stanovení
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DAVID Marek DUBOVÝ Petr HRADILOVÁ SVÍŽENSKÁ Ivana KLUSÁKOVÁ Ilona

Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Popis Events in the spinal nerve roots after distal peripheral nerve injury, which induces neuropathic pain, are little characterized. Our previous results from immunofluorescence (IF) microscopy and computer-assisted image analysis revealed endoneurial extracellular matrix remodulation following dorsal and ventral rhizotomy. Therefore, we performed a quantitative immunohistochemical analysis to detect changes of laminin-1 (lam-1) in the ipsi- and contralateral spinal nerve roots of two neuropathic pain models - sciatic (ScNL) and spinal nerve (SNL) ligature. Behavioral tests demonstrated neuropathic pain induction (mechanical allodynia and thermal hyperalgesia) in ipsilateral hind paws of all experimental animals. A higher IF intensity of lam-1 was found in the ventral compared to dorsal roots of naive rats. In contrast to naive rats, significant bilateral enhancement of lam-1 IF was detected in the dorsal and ventral roots 1 to 3 days after ScNL as well as 1 to 7 days after SNL. Endoneurial lam-1 IF intensity in both ipsi- and contralateral spinal nerve roots was decreased 7 days following ScNL and 14 days from SNL. The decreased lam-1 IF intensity in the endoneurium of spinal nerve roots was to the level of IF brightness measured in naive animals. Even if endoneurial lam-1 IF changes are more distinct on ipsi- than contralateral side, their patterns in contralateral spinal nerve roots coincided with ipsilateral ones after both types of unilateral nerve ligature. Generally, lam-1 IF changes are larger in ScNL than SNL model of neuropathic pain. In conclusion, both proximal and distal nerve injuries lead to remodulation of the endoneurial lam-1 in the spinal nerve roots.
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