Informace o publikaci

An Electrochemical Study of DNA and RNA Short Fragments by Elimination Voltammetry

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TRNKOVÁ Libuše JELEN František BALCAROVÁ Zdeňka NEPLECHOVÁ Kamila

Rok publikování 2010
Druh Další prezentace na konferencích
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis Short oligonucleotides play an important role in many biological processes including triplet repeat expansion associated with neurodegenerative diseases (fragile X syndrome, Huntington’s disease, Friedreich’s ataxia or myoclonic epilepsy) [1]. One of the most stable DNA fragments, which forms hairpin, is heptamer d(GCGAAGC) [2]. In our electrochemical study we analyzed not only this hairpin but also DNA and RNA fragments containing self-complementary nucleobases (GC) forming the stem. Both DNA and RNA analogues adsorbed on mercury electrodes provided voltammetric reduction signals of adenine and cytosine (A and C) and oxidation signals of guanine (G). We found significant differences between DNA and RNA fragments depending on their length, sequence, and pH (Fig. 1). For the resolution of A and C reduction signals and evaluation of the nature of anodic processes of the G signal in both DNA and RNA fragments, elimination voltammetry with linear scan (EVLS) was utilized [3,4].
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