A new approach how to define coefficient of electroactivity of adenine and its twelve derivatives using flow injection analysis with amperometric detection.

• Autoři: ZÍTKA Ondřej; SKUTKOVA Helena; ADAM Vojtěch; TRNKOVÁ Libuše; BABULA Petr; HUBÁLEK Jaromír; PROVAZNÍK Ivo; KIZEK René
• Rok publikování: 2011
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Electroanalysis
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• www: http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=17&SID=V254NakJe2LpBEPl4g1&page=1&doc=1
• Doi: http://dx.doi.org/10.1002/elan.201100064
• Obor: Fyzikální chemie a teoretická chemie
• Klíčová slova: Adenine derivatives; Flow injection analysis; Amperometric detection; Glassy carbon electrode; Electroactivity
• Popis: We studied the electrochemical behaviour of adenine derivates (adenosine, 2-aminopurine, 2,6-diaminopurine, 6-benzyl-aminopurine, adenosine monophosphate, cyclic adenosine monophosphate, nicotinamide adenine dinucleotide, adenosine triphosphate, S-adenosyl-L-methionine, and synthetic derivatives AD-3, AD-6 and AD-9) using flow injection analysis with electrochemical detection using a glassy carbon electrode. The influences of pH, flow rate and potential on the signal height of the studied derivates were tested. The optimal pH was 3, the flow rate of the mobile phase 0.75 mLmin(-1) and the potential 1100 mV. Further, we attempted to characterize each of the studied derivatives by mathematical equations and classic analytical parameters. The lowest detection limit was estimated for adenine as 0.9 nM and 2-aminopurine as 0.5 nM.

Související projekty

• Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni