BCR-ABL activity measured by 50% inhibitory concentration for imatinib, p-CrkL/CrkL ratio or p-CrkL ratio in CD34+ cells of patients with chronic myeloid leukemia does not predict treatment response.
|Autoři||ŠIMARA Pavel — PETERKOVÁ Martina — STEJSKAL Stanislav — POTĚŠILOVÁ Michaela — KRONTORÁD KOUTNÁ Irena — RÁČIL Zdeněk — RÁZGA Filip — JURČEK Tomáš — DVOŘÁKOVÁ Dana — MAYER Jiří|
|Druh||Článek v odborném periodiku|
|Obor||Onkologie a hematologie|
|Klíčová slova||p-CrkL; chronic myeloid leukemia; imatinib|
In this study, we assessed BCR-ABL kinase activity by measuring the protein levels of CrkL and its phosphorylated form (p-CrkL) in order to predict the clinical outcome of newly diagnosed chronic myeloid leukemia patients. CD34+ cells from these patients were collected before the start of imatinib therapy and treated in vitro with imatinib. The reduction of p-CrkL and CrkL protein levels was then measured by flow cytometry. The data were processed using three independent approaches: an assessment of a) IC50imatinib, b) p-CrkL/CrkL ratio, and c) p-CrkL ratio. The results were subsequently correlated with the clinical response of patients after 3 and 6 months of imatinib therapy. None of the three p-CrkL parameters measured in CD34+ cells was found to be predictive of clinical outcome.
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