Publication details

A new F-actin structure in fungi: actin ring formation around the cell nucleus of Cryptococcus neoformans

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Authors

KOPECKÁ Marie KAWAMOTO Susumu YAMAGUCHI Masashi

Year of publication 2013
Type Article in Periodical
Magazine / Source Journal of Electron Microscopy
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1093/jmicro/dfs074
Field Genetics and molecular biology
Keywords perinuclear F-actin ring; nucleus; Cryptococcus neoformans
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Description The F-actin cytoskeleton of Cryptococcus neoformans is known to comprise actin cables, cortical patches and cytokinetic ring. Here, we describe a new F-actin structure in fungi, a perinuclear F-actin collar ring around the cell nucleus, by fluorescent microscopic imaging of rhodamine phalloidin-stained F-actin. Perinuclear F-actin rings form in Cryptococcus neoformans treated with the microtubule inhibitor Nocodazole or with the drug solvent dimethyl sulfoxide (DMSO) or grown in yeast extract peptone dextrose (YEPD) medium, but they are absent in cells treated with Latrunculin A. Perinuclear F-actin rings may function as ‘funicular cabin’ for the cell nucleus, and actin cables as intracellular ‘funicular’ suspending nucleus in the central position in the cell and moving nucleus along the polarity axis along actin cables.
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