Publication details

Effect of sarcosine on antioxidant parameters and metallothionein content in the PC-3 prostate cancer cell line

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Authors

CERNEI Natalia Vladimirovna ZÍTKA Ondřej SKALICKOVA Sylvie GUMULEC Jaromír SZTALMACHOVÁ Markéta RODRIGO Miguel Angel Merlos SOCHOR Jiří MASAŘÍK Michal ADAM Vojtěch HUBÁLEK Jaromír TRNKOVÁ Libuše KRUSEOVA Jarmila ECKSCHLAGER Tomáš KIZEK René

Year of publication 2013
Type Article in Periodical
Magazine / Source Oncology Reports
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.3892/or.2013.2389
Field Biochemistry
Keywords sarcosine; prostate cancer; metallothionein; antioxidant activity; chip electrophoresis; ion-exchange chromatography; Brdicka reaction
Attached files
Description Sarcosine is currently one of the most discussed markers of prostate cancer. It is involved in amino acid metabolism and methylation processes that occur during the progression of prostate cancer. In this study, we monitored the effect of the addition of sarcosine (0; 10; 250; 500; 1,000 and 1,500 mu M) in a time-dependent manner (0-72 h) on the PC-3 prostate cancer cell line. For the assessment of cell viability, the commonly used MTT test was employed. Furthermore, ion-exchange liquid chromatography was used for the determination of sarcosine content in the PC-3 cells. We also determined metallothionein (MT) levels by chip capillary electrophoresis and Brdicka reaction in the cells treated with sarcosine. Sarcosine levels in the cells increased in a concentration-dependent manner levels increased from only 270 nM with the lowest applied concentration of sarcosine (10 mu M) to 106 mu M with the highest applied concentration of sarcosine (1,500 mu M). There was a marginal change observed in the MT concentration. Finally, the antioxidant activity of the PC-3 cells was determined using five different spectrophotometric methods [2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing ability of plasma (FRAP), free radicals, N,N-dimethyl-p-phenylenediamine (DMPD) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS)]. A significant negative correlation was observed between DPPH and FRAP (r=-0.68 at P<0.001) and between DMPD and ABST (r=-0.64 at P<0.001). Additionally, as regards the correlation between MT and DPPH, a significant positive trend (r=0.62 at P<0.001) was observed.
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