Publication details

IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING

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Authors

CHUDOBOVA Dagmar CIHALOVA Kristyna GURÁŇ Roman DOSTALOVA Simona SMERKOVA Kristyna VESELÝ Radek GUMULEC Jaromír HEGER Zbynek MASAŘÍK Michal ADAM Vojtěch KIZEK René

Year of publication 2014
Type Article in Proceedings
Conference CECE 2014: 11th International Interdisciplinary Meeting on Bioanalysis
MU Faculty or unit

Faculty of Medicine

Citation
Web http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf
Field Genetics and molecular biology
Keywords HARD-TO-HEAL WOUNDS; MALDI-TOF MS; SEQUENCING
Description This study is focused on the isolation, characterization and identification of the most abundant microorganisms in superficial wounds of patients (n = 50) suffering from bacterial infection. Primarily, the identification of the strains was carried out through Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) based on comparison of protein profiles (2-30 kDa) with database. Sanger sequencing of 16S bacterial gene was used for confirmation if the score was < 2.
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