Publication details

Note: Non-invasive optical method for rapid determination of alignment degree of oriented nanofibrous layers

Authors

POKORNÝ Marek KLEMEŠ Jan ŘEBÍČEK Jiří KOTZIANOVÁ Adéla VELEBNÝ Vladimír

Year of publication 2015
Type Article in Periodical
Magazine / Source Review of Scientific Instruments
MU Faculty or unit

Faculty of Science

Citation
Web http://scitation.aip.org/content/aip/journal/rsi/86/10/10.1063/1.4935021
Doi http://dx.doi.org/10.1063/1.4935021
Field Physical chemistry and theoretical chemistry
Keywords TISSUE-ENGINEERING SCAFFOLDS; FIBER ALIGNMENT; ANISOTROPY; DESIGN
Attached files
Description This paper presents a rapid non-destructive method that provides information on the anisotropic internal structure of nanofibrous layers. A laser beam of a wavelength of 632.8 nm is directed at and passes through a nanofibrous layer prepared by electrostatic spinning. Information about the structural arrangement of nanofibers in the layer is directly visible in the form of a diffraction image formed on a projection screen or obtained from measured intensities of the laser beam passing through the sample which are determined by the dependency of the angle of the main direction of polarization of the laser beam on the axis of alignment of nanofibers in the sample. Both optical methods were verified on Polyvinyl alcohol (PVA) nanofibrous layers (fiber diameter of 470 nm) with random, single-axis aligned and crossed structures. The obtained results match the results of commonly used methods which apply the analysis of electron microscope images. The presented simple method not only allows samples to be analysed much more rapidly and without damaging them but it also makes possible the analysis of much larger areas, up to several square millimetres, at the same time.

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