Publication details

MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus

Investor logo
Authors

HÉŽOVÁ Renata KOVAŘÍKOVÁ Alena SROVNAL Josef ZEMANOVÁ Milada HARUSTIAK Tomáš EHRMANN Jiří HAJDÚCH Marian ŠACHLOVÁ Milana SVOBODA Marek SLABÝ Ondřej

Year of publication 2016
Type Article in Periodical
Magazine / Source Tumor Biology
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://link.springer.com/article/10.1007%2Fs13277-015-4656-8
Doi http://dx.doi.org/10.1007/s13277-015-4656-8
Field Oncology and hematology
Keywords Esophageal adenocarcinoma; Esophageal squamous cell carcinoma; miR-205; Tumor suppressor; Oncogene
Description Esophageal cancer is a malignant disease with poor prognosis, increasing incidence, and ineffective treatment options. MicroRNAs are post-transcriptional regulators of gene expression involved in many biological processes including carcinogenesis. We determined miR-205 expression levels in tumor/non-tumor tissues of 45 esophageal cancer patients using qPCR and found that decreased level of miR-205 in tumor tissue correlates with poor overall survival in esophageal adenocarcinoma patients. Further, we observed significantly higher levels of miR-205 in tumor tissue of esophageal squamous cell carcinoma. Ectopic overexpression of miR-205 in adenocarcinoma cell line SK-GT-4 led to decreased cell proliferation, cell cycle arrest in G1, and decreased migration ability. Conversely, in squamous cell line KYSE-150, same effects like inhibition of proliferation, migration, and colony-forming potential and cell cycle arrest in G2 were observed after silencing of miR-205. We performed global gene expression profiling and revealed that suppressive functioning of miR-205 in adenocarcinoma could be realized through regulation of epithelial-mesenchymal transition (EMT), whereas oncogenic in squamous cell carcinoma by regulation of metalloproteinase 10. Our results suggest that miR-205 could serve as biomarker in esophageal cancer and acts as a tumor suppressor in esophageal adenocarcinoma and oncogene in esophageal squamous cell carcinoma.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info