Publication details
Glucose determination in fish plasma by two different moderate methods
| Authors | |
|---|---|
| Year of publication | 2016 |
| Type | Article in Periodical |
| Magazine / Source | Acta Veterinaria |
| MU Faculty or unit | |
| Citation | |
| Web | http://dx.doi.org/10.2754/avb201685040349 |
| Doi | http://dx.doi.org/10.2754/avb201685040349 |
| Field | Physiology |
| Keywords | glucometer; spectrophotometry; tench; Tinca tinca |
| Description | Group of teleost fish consist from many different species and has large economic, societal and environmental impact. Glucose is the main source of energy for most vertebrate organisms. Fish as well as for example mammals maintain specific level of blood sugar – glycaemia, which is influenced by multiple factors such as hormone production, diet and temperature. Therefore, it is favourable parameter of scientists to check the immediate state of an organism. Most utilized method to measure glycaemia in farmed fish is spectrophotometry, however for field experiments is this method often inconvenient. Here we report that basic spectrophotometry using oxidation of blood beta D-glucose by glucose oxidase (used in laboratory conditions) can be easily substituted with portable glucometer measurements, primarily designed for human samples. This is very useful thanks to the possibility of use outside the laboratory and its small sample volume demands. Terrain studies often require fast sample processing and conditions of measurement are limited. Using glucometer is very simple and elegant option. We have tested 74 blood plasma samples (29 males, 45 females) of tench (Tinca tinca) both spectrophotometrically and using human glucometer. Our results show significant linear correlation (p < 0.001) between both methods. The main contribution of this study consists in comparability and interchangeability of results obtained from both presented methods. |