Publication details

FZD(10)-G alpha(13) signalling axis points to a role of FZD(10) in CNS angiogenesis

Investor logo
Authors

HOT B. VALNOHOVA J. ARTHOFER E. SIMON K. SHIN J. UHLEN M. KOSTENIS E. MULDER J. SCHULTE Gunnar

Year of publication 2017
Type Article in Periodical
Magazine / Source Cellular Signalling
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1016/j.cellsig.2017.01.023
Keywords WNT; FZD; GPCR; Endothelial cell; Angiogenesis; GNA13; YAP/PAZ
Description Among the 10 Frizzled (FZD) isoforms belonging to the Class F of G protein-coupled receptors (GPCRs), FZD(10) remains the most enigmatic. FZD10 shows homology to FZD(4) and FZD(9) and was previously implicated in both beta-catenin-dependent and-independent signalling. In normal tissue, FZD(10) levels are generally very low; however, its upregulation in synovial carcinoma has attracted some attention for therapy. Our findings identify FZD(10), as a receptor interacting with and signalling through the heterotrimeric G protein G alpha(13) but not G alpha(12), G alpha(i1,) G alpha(oA), G alpha(s), or G alpha(q). Stimulation with the FZD agonist WNT induced the dissociation of the G alpha(13) protein from FZD(10), and led to global G alpha(12/13)-dependent cell changes assessed by dynamic mass redistribution measurements. Furthermore, we show that FZD(10) mediates G alpha(12/13) activation-dependent induction of YAP/TAZ transcriptional activity. In addition, we show a distinct expression of FZD(10) in embryonic CNS endothelial cells at E11.5-E14.5. Given the well-known importance of G alpha(13) signalling for the development of the vascular system, the selective expression of FZD(10) in brain vascular endothelial cells points at a potential role of FZD(10)-G alpha(13) signalling in CNS angiogenesis. (C) 2017 Elsevier Inc. All rights reserved.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info