Publication details

Relationship of epigenetic variability of miR-124 to extracellular matrix remodelling and age-related MMP-3 expression in rheumatoid arthritis

Authors

PÁVKOVÁ GOLDBERGOVÁ Monika LIPKOVÁ Jolana FEDORKO Jan NĚMEC Petr GATTEROVÁ Jindřiška VÁLKOVÁ Lucie VEVERKOVÁ Jana VAŠKŮ Anna

Year of publication 2018
Type Article in Periodical
Magazine / Source General physiology and biophysics
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.4149/gpb_2018024
Keywords Rheumatoid arthritis; Protein; microRNA; Expression; MMP
Description The aim of study was to examine relation among miR-124 and serum levels of selected cytokines and chemokines, MMP-3, production of auto-antibodies, and factors describing clinical activity (DAS28) and radiographic progression in rheumatoid arthritis (RA). A total of 80 RA patients according to the ACR classification criteria, and 32 control subjects were recruited into study. The measurements of miR-124 and U-6 expression, CRP anti-CCP, rheumatoid factors (RFs), radiographs of both hands with calculation of total sharp score (TSS), DAS28 and cytokines, chemokines and MMP levels in serum were obtained from all RA patients. miR-124 was down-regulated in RA patients compared to controls (7-fold decrease). The miR-124 expression correlated to MMP-3 levels (p < 0.001), which were in multivariate analysis associated to age of RA onset. Higher levels were detected in younger subjects. No relation of miR-124 expression to measures of RA activity (DAS28 score; TSS), auto-antibodies (anti-CCP, RR, RF IgG, RF IgA, RF IgM), acute inflammatory markers (CRP, IL-6), and other cytokine and chemokines (IL-13, IL-15, IL-8, TNF-alpha, MCP-1, RANTES) was observed. In conclusion, we present a down-regulation of miR-124 in RA patients and its correlation to MMP-3 levels, which associated to age of RA onset.
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