Publication details
Paenibacillus farraposensis sp. nov., isolated from a root nodule of Arachis villosa
| Authors | |
|---|---|
| Year of publication | 2022 |
| Type | Article in Periodical |
| Magazine / Source | International journal of systematic and evolutionary microbiology |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005294 |
| Doi | http://dx.doi.org/10.1099/ijsem.0.005294 |
| Keywords | Paenibacillus; root nodule bacteria; root colonization; Paenibacillus polymyxa complex; biocontrol |
| Description | Strain UY79T was isolated from a root nodule of Arachis villosa, collected at the Esteros de Farrapos National Park, Río Negro, Uruguay. Cells were non-motile Gram-variable rods with central to subterminal oval to ellipsoidal endospores that swell the sporangia. Growth was observed in the range of 15–42?°C (optimum, 30?°C), pH 5.0–9.0 (optimum, pH 7.0–8.0) and with up to 3?% (w/v) NaCl (optimum, 1–2?%). Strain UY79T was facultative anaerobic, catalase-positive and oxidase-negative. According to the results of 16S rRNA gene sequence analysis, UY79T belongs to the genus Paenibacillus and is closely related to P. ottowii MS2379T, P. peoriae BD-57T, P. polymyxa ATCC 842T and P. brasilensis PB172T, exhibiting 99.4, 99.0, 99.0 and 98.9% sequence identity, respectively. Average nucleotide identity and digital DNA–DNA hybridization values with the most closely related type strains were 74.3–88.6% and 38.2–48.7?%, respectively. Major fatty acids (>10?%) were anteiso-C15:0, iso-C15:0, and C16?:?0. Menaquinones MK-7 and MK-6 were the only isoprenoid quinones detected. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid. Spermidine was the predominant polyamine. The DNA G+C content based on the draft genome sequence was 46.34?mol%. Based on the current polyphasic study, UY79T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus farraposensis sp. nov. is proposed. The type strain is UY79T (=CCM 9147T=CGMCC 1.19038T). |