Publication details

Droplet-Based Microfluidic Temperature-Jump Platform for the Rapid Assessment of Biomolecular Kinetics

Investor logo
Investor logo
Authors

YANG Tianjin VILLOIS Alessia KUNKA Antonín GRIGOLATO Fulvio AROSIO Paolo PROKOP Zbyněk DEMELLO Andrew STAVRAKIS Stavros

Year of publication 2022
Type Article in Periodical
Magazine / Source Analytical chemistry
MU Faculty or unit

Faculty of Science

Citation
Web https://doi.org/10.1021/acs.analchem.2c03009
Doi http://dx.doi.org/10.1021/acs.analchem.2c03009
Keywords Biotechnology; Fluid dynamics; Kinetics; Liquids; Nanofibers
Description Protein folding, unfolding, and aggregation are important in a variety of biological processes and intimately linked to “protein misfolding diseases”. The ability to perform experiments at different temperatures allows the extraction of important information regarding the kinetics and thermodynamics of such processes. Unfortunately, conventional stopped-flow methods are difficult to implement, generate limited information, and involve complex sample handling. To address this issue, we present a temperature-controlled droplet-based microfluidic platform that allows measurement of reaction kinetics on millisecond to second timescales and at temperatures between ambient and 90 °C. The utility of the microfluidic platform for measuring fast biomolecular kinetics at high temperatures is showcased through the investigation of the unfolding kinetics of haloalkane dehalogenases and the elongation of fibrils composed of the amyloid ß peptide associated with Alzheimer’s disease. In addition, a deep-ultraviolet (UV) fluorescence microscope was developed for the on-chip recording of protein intrinsic fluorescence spectrum originating from aromatic amino acid residues. We envision that the developed optofluidic platform will find wide applicability in the analysis of biological processes, such as protein refolding and phase separation.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info