Publication details

Dynamics of b-glucosidase Zm-p60.1 ectopic expression during transgenic pollen development: a histochemical approach

Authors

DUBOVÁ Jaroslava MLEJNEK Petr BRZOBOHATÝ Břetislav

Year of publication 1999
Type Article in Periodical
Magazine / Source General Physiology and Biophysics
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords indigogenic method; beta-glucosidase; tobacco; pollen
Description Indigogenic method was used for histochemical localization of Zm-p60.1 (cytokinin-glucoside specific)beta-glucosidase activity in various developmental stages of transgenic tobacco anthers. Expression of Zm-p60.1 cDNA was controlled by two different promoters (CaMV 35S, LAT 52). Histochemical detection has proved different patterns of beta-glucosidase activity during pollen development in these two types of transformants.
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