Publication details

Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase.

Authors

CHROUST Karel JOWETT Trevor FARID-WAJIDI Mustafa HUANG Juan RYŠKOVÁ Martina WOLF Ronald HOLOUBEK Ivan

Year of publication 2000
Type Article in Periodical
Magazine / Source Mutation Research
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Transgenic Drosophila melanogaster; wing spot test; female sterility; aneuploidy; glutathione S-transferase; 1;2:5;6-dibenzanthracene; 1;2-dichloroethane
Description Sensitivity of transgenic Drosophila melanogaster with expression of a human gene encoding the glutathione S-transferase alpha subunit (GSTA1-1) to 1,2:5,6-dibenzanthracene (DBA) and 1,2-dichloroethane (DCE) was investigated in the somatic mutation and recombination test (SMART). We performed the same assay in control transgenic flies expressing the bacterial lacZ gene. Three types of transgenic Drosophila strains carrying GSTA1-1 were used: two transgenic strains homozygous for the second chromosome with a single-copy transgene insertion and one strain with two transgene insertions. Larvae carrying the lacZ gene were significantly more sensitive to genotoxic effects of DBA than those carrying three copies of the GSTA1-1 gene. The larvae with lacZ expression showed significantly lower sensitivity to DCE compared with those expressing GSTA1-1. Finally, a pretreatment with buthionine-sulphoximine (BSO) in experiment with DCE significantly decreased the frequency of mutation events in larvae with three GSTA1-1 copies in comparison with others.
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