Publication details

Use of enzymes and proteins immobilized on magnetic polymer particles in bacterial molecular diagnostics

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Authors

ŠPANOVÁ Alena RITTICH Bohuslav PRODĚLALOVÁ Jana HORÁK Daniel LENFELD Jiří BENEŠ Milan J.

Year of publication 2001
Type Article in Proceedings
Conference Separation in the BioSciences SBS2001
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords magnetic cellulose; magnetic poly(HEMA-co-EDMA); enzymes; immobilization; applications in microbiology
Description Use of different enzymes and proteins immobilized on hydrophilic magnetic carriers - bead cellulose and poly(HEMA-co-EDMA) was demosnstrated in different applications: digestion of high-molecular weight RNA, chromosomal DNAs, introduction of single and double-stranded nicks into plasmid DNAs, degradation of bacterial intracellular PCR inhibitors, immunomagnetic separation of bacterial cells from food samples.
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