Publication details

Genetic variation in the promoter region of the basic Fibroblast Growth Factor gene (bFGF)

Authors

BERÁNEK Michal TSCHÖPLOVÁ Svatava KAŇKOVÁ Kateřina KUHROVÁ Viera VÁCHA Jiří

Year of publication 2002
Type Article in Periodical
Magazine / Source Human Immunology
MU Faculty or unit

Faculty of Medicine

Citation
Field Genetics and molecular biology
Keywords bFGF; genetic polymorphism; linkage disequilibrium
Description Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor family that possess broad mitogenic and cell survival activities and is involved in a variety of biological processes. We investigated possible genetic polymorphism in the promoter and 5 flanking region of the bFGF gene. Polymorphism was analysed by means of heteroduplex analysis, fragments with altered mobility were sequenced. Three novel substitutions (-553T/A, -834T/A and -921C/G) were identified in the promoter region. Allele frequencies in a sample of healthy Caucasian subjects (n=126) were determined by polymerase chain reactions followed by restriction analyses with specific endonucleases. The frequencies of the mutated alleles (-553A, -834A and 921G) were 0.04, 0.05 and 0.14, respectively. Newly identified variants in the bFGF gene promoter appear to be common polymorphisms in the Czech population.
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