Publication details

Inhibition study of rhodanese by means of electrophoretically mediated microanalysis

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Authors

NOVÁKOVÁ Soňa TELNAROVÁ Magdalena GLATZ Zdeněk

Year of publication 2003
Type Article in Periodical
Magazine / Source Journal of Chromatography A
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Capillary electrophoresis; Rhodenase; EMMA; Inhibition
Description A combination of the electrophoretically mediated microanalysis methodology with a partial filling technique was applied for the inhibition study of the bovine liver rhodanese by 2-oxoglutarate. In this set-up, the part of capillary is filled with the buffer best for the enzymatic reaction whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. The estimated value of KI for 2-oxoglutarate was 3.62 x 10-4 ą 1.43 x 10-4 M with respect to cyanide and 1.40 x 10-3 ą 1.60 x 10-4 M with respect to thiosulfate. Moreover the type of inhibition was also evaluated. The findings of 2-oxogluatarate as the competitive inhibitor with respect to cyanide and as the uncompetitive inhibitor with respect to thiosulfate are in accordance with previous literature data.
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