Publication details

PIN proteins perform a rate-limiting function in cellular auxin efflux

Authors

PETRÁŠEK J. MRAVEC J. BOUCHARD R. BLAKESLEE J. ABAS M. SEIFERTOVÁ D. WISNIEWSKA J. TADELE Z. ČOVANOVÁ M. DHONUKSHE P. SKŮPA P. BENKOVÁ E. PERRY L. KŘEČEK P. LEE O.R. FINK G. GEISLER M. MURPHY A. LUSCHNIG C. ZAŽÍMALOVÁ E. FRIML J.

Year of publication 2006
Type Article in Periodical
Magazine / Source Science
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords auxin transport; auxin efflux; PIN proteins; Arabidopsis
Description Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific PIN proteins and several PGP transporters are crucial factors in auxin transport-related development, yet PINs molecular function remains unknown. Here we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info