Publication details

Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis

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Authors

DAVID Marek GABRIEL Miroslav KOPECKÁ Marie

Year of publication 2007
Type Article in Periodical
Magazine / Source Cell Biology International
MU Faculty or unit

Faculty of Medicine

Citation
Web
Field Other medical specializations
Keywords actin; basidiomycetous yeasts; cytoskeleton; Malassezia pachydermatis; microtubules; ultrastructure
Description Microtubular and actin cytoskeletons were investigated by fluorescence and electron microscopy. Microtubules were detected by indirect immunofluorescence. Cytoplasmic microtubules were detected in interphase and a spindle with astral microtubules was seen in M-phase. The disintegration of cytoplasmic microtubules and migration of the nucleus to the bud before mitosis were characteristic features of the basidiomycetous yeast Malassezia pachydermatis. The visualisation of F-actin structures (patches, cables and cytokinetic rings) by fluorescence microscopy using both monoclonal anti-actin antibody and rhodamine-phalloidin failed, but actin was detected by electron microscopy with immunogold labelling. Clusters of gold particles indicating actin structures were detected at the plasma membrane of cells with unique cortical ultrastructural features characteristic of the genus Malassezia. A possible association of these with the actin cytoskeleton is suggested.
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