Comparison of PCR-ready DNA Isolation Methods from Lactic Acid Bacteria used in Dairy Industry

• Authors: ŠPANOVÁ Alena; RITTICH Bohuslav; NĚMCOVÁ Eva; KŘÍŽOVÁ Jana; HORÁK Daniel
• Year of publication: 2008
• Type: Article in Proceedings
• Conference: 7th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
• MU Faculty or unit: Faculty of Science
• Field: Genetics and molecular biology
• Keywords: DNA; isolation; magnetic microspheres; lactic acid bacteria; PCR
• Description: Different methods for isolation of PCR-ready DNA were tested. The influence of the DNA isolation procedures tested on the reproducibility of RAPD and rep-PCR was studied. The reproducibility of amplification of DNA isolated by the salting-out method and adsorption on magnetic nonporous microspheres was comparable with the phenol extraction method.

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