Application of polyacrylamide GE and MS for antifungal proteins in the barley and malt caryopsis

• Authors: HÉGROVÁ Blanka; FARKOVÁ Marta; MACUCHOVÁ Simona; HAVEL Josef; PREISLER Jan
• Year of publication: 2008
• Type: Article in Proceedings
• Conference: CECE 2008, 5th international interdisciplinary meeting on bioanalysis
• MU Faculty or unit: Faculty of Science
• Field: Analytic chemistry
• Keywords: antifungal proteins - barley - malt - PAGE - MS - chemometric evaluation
• Description: Plants are exposed to a large array of phytopathogenic organisms and the survival in these conditions demands quick defense responses which include the synthesis of defense antifungal proteins - hordothionins (HTs) - associated with deggre of beer gushing. RIBM data and data observed from GE and MS were chemometric evaluated by program Statistica, V8.0. The presence of HT observed in the barley and malt caryopsis does not correlate with gushing observed in malt caryopsis (|R| belons to interval 0.07, 0.34).

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