Publication details

Variability of soil microbial properties: effects of sampling, handling and storage

Authors

ČERNOHLÁVKOVÁ Jitka JARKOVSKÝ Jiří NEŠPOROVÁ Michala HOFMAN Jakub

Year of publication 2009
Type Article in Periodical
Magazine / Source Ecotoxicology and Environmental Safety
MU Faculty or unit

Faculty of Science

Citation
Field Soil contamination adn decontamination incl. pesticides
Keywords Soil biomonitoring; microbial biomass; respiration
Description We investigated the effect of soil spatial variability within the sampling site scale, the effects of sample sieving (1, 2 and 4 mm), and storage conditions up to 32 weeks (wet at 4 C, -20 C and air dried) on microbial biomass C, respiration, ammonification and nitrification activities in arable, grassland and forest soil. In general, all results were dependent on soil type. Arable soil showed the highest spatial variability, followed by grassland and forest soil. Sieving did not cause large differences, however higher biomass C and respiration activity were observed in 1 mm than in 4 mm fraction. Storage at 4 C seemed to be the most appropriate up to 8 weeks showing only minor changes of microbial parameters. Freezing of soils resulted in large increase of respiration. Dried storage indicated disruption of microbial communities even after 2 weeks.
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