LC-MS/MS Monitoring Human Plasma Proteins Potentionally related to Graft-versus-Host Disease

• Authors: JEDLIČKOVÁ Lenka; BRZOBOHATÝ Jiří; KABÁTHOVÁ Jitka; VERNER Jan; POSPÍŠILOVÁ Šárka; ZDRÁHAL Zbyněk
• Year of publication: 2010
• Type: Conference abstract
• MU Faculty or unit: Faculty of Medicine
• Description: The aim of this study was to develop a reliable and sensitive method for determination of potential biomarkers in human plasma for early GVHD detection. Blood plasma was immunodepleted by MARS Hu-14 to remove fourteen most abundant proteins. The immunodepleted plasma samples were fractionated by LC capillary system on a reverse phase (mRP C18, Agilent) in water/acetonitrile gradient and subsequently digested with trypsin. The resulting peptides were analyzed using LC-MS/MS in autoMS/MS mode.

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